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Deoxyribonuclease I
Source: Bovine Pancrease
EC: 3.1.21.1
DESCRIPTION
  Bovine pancreatic deoxyribonuclease (DNase) is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with free hydroxyl group at the 3' position and phosphate group at the 5' position. The optimum pH is 7.8. DNase I is activated by bivalent metals, and inhibited by chelating agents such EDTA and sodium dodecyl sulfate. DNase I can be stabilized against proteolytic digestion by 5 mM Ca2+.
Deoxyribonuclease has been chromatographically purified to remove trace contaminating proteases. DNase may be used in clinic to thin and liquefy mucus in the lungs.
SPECIFICATION (I)

Item

Specifications

Methods

Appearance

White or pale yellow lyophilized powder

 

Activity

≥ 3,000 Christensen units/mg powder

K.P.

Loss on drying

≤ 0.3%

K.P.

Residue on ignition

≤ 1.0%

K.P.


Conclusion: Product complies with KP.
SPECIFICATION (II)
Activity ≥ 500 Kunitz units/mg powder Sigma-aldrich
SPECIFICATION (III)
Activity ≥ 2,000 Kunitz units/mg powder Sigma-aldrich
STORAGE
Store in an airtight container, at a temperature of 2°C to 8°C
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