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| Deoxyribonuclease I
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Source: Bovine Pancrease
EC: 3.1.21.1
DESCRIPTION
Bovine pancreatic deoxyribonuclease (DNase) is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with free hydroxyl group at the 3' position and phosphate group at the 5' position. The optimum pH is 7.8. DNase I is activated by bivalent metals, and inhibited by chelating agents such EDTA and sodium dodecyl sulfate. DNase I can be stabilized against proteolytic digestion by 5 mM Ca2+.
Deoxyribonuclease has been chromatographically purified to remove trace contaminating proteases. DNase may be used in clinic to thin and liquefy mucus in the lungs.
SPECIFICATION (I)
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Item
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Specifications
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Methods
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Appearance
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White or pale yellow lyophilized powder
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Activity
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≥ 3,000 Christensen units/mg powder
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K.P.
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Loss on drying
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≤ 0.3%
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K.P.
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Residue on ignition
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≤ 1.0%
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K.P.
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Conclusion: Product complies with KP.
SPECIFICATION (II)
Activity ≥ 500 Kunitz units/mg powder Sigma-aldrich
SPECIFICATION (III)
Activity ≥ 2,000 Kunitz units/mg powder Sigma-aldrich
STORAGE
Store in an airtight container, at a temperature of 2°C to 8°C
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| Next:Ribonuclease A |
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